Các trình tự gen trên GenBank

1. Hoang,T.T.H., Nguyen,T.N.L., Le,V.S. and Chu,H.M. (2018) Aconitum carmichaelii isolate HSP internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MH410519.1. 19; 06-JUL-2018
2. Hoang,T.T.H., Nguyen,T.N.L., Le,V.S. and Chu,H.M. (2018) Aconitum carmichaelii isolate QB internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MH410520.1. ; 06-JUL-20183. Hoang, T,T.H., Nguyen, T,N.L., Le, V,S and.Chu. and H,M. (2018)  Aconitum carmichaelii plastid:chloroplast genomic DNA containing MatK gene region, isolate QB, Hagiang. GenBank: LS398143.1.
3. Code number: ĐH2017-TN04-04
4. Coordinator: Dr. Nguyen Thi Ngoc Lan
5. Implementing institution: Thai Nguyen University of Education
6. Duration: 24 months.

2. Objective(s)

Analysis of morphological characteristics, classification and construction of in vitro culture process in order to contribute to the conservation and development of the gene source of Aconitum carmichaelii Debx.

3. Creativeness and innovativeness

• Based on a combination of the morphological characteristics and the nucleotide sequences of ITS and matK gene fragment, the Aconitum samples collected at Hagiang were identified as Aconitum carmichaeli Debx.
• An in vitro culture procedure has been developed from the stem segments of Aconitum carmichaeli plants.
• A hairy root culture procedure has been developed from the root segments of the in vitro Aconitum carmichaelii plants.

4. Research results

• Aconitum samples collected from Hoang Su Phi and Quan Ba districts, Ha Giang were identified as A. carmichaelii species based on a combination of morphological, anatomical and nucleotide sequences of ITS and matK.
• The nodal stem segment is a suitable material for in vitro multi-shoot induction of the A. carmichaelii. The optimal medium for multi-shoot induction was MS + 30 g L^-1 saccharose + 9 g L^-1 agar + 1.5 mg L^-1 BAP, with 4.57 shoots per explants after 8 weeks. Basic MS medium supplemented with 1.0 mg L^-1 Kin induced a lower number of shoots, at only 3.80 shoots per explant. The highest number of roots after 8 weeks of culture was achieved on MS medium supplemented with 0.5 mg L^-1 IBA, at 3.60 ± 0.16 (roots / shoot), while it was 3.07 ± 0.07 (roots /shoot) on MS medium supplemented with 0.7 mg L^-1 α-NAA.
• Using A. rhizogenes-mediated transformation of in vitro A. carmichaeli root segments, hairy roots were produced on MS+ 30 g L^-1 saccharose + 500 mg L^-1 cefotaxime +100 μmol L^-1 AS. The maximum fresh weight of hairy roots is the highest (3.22 g of fresh weight) in liquid medium under shaking conditions and after 6 weeks, the hairy root mass produced 5.85-fold compared to the initial mass.

5. Products

5.1. Journal papers

1. Quan Huu Nguyen, Hoan Thi Thu Hoang, Hong Thi Tran, Thuy Thi Thu Vu, Thuong Danh Sy, Mau Hoang Chu, Lan Thi Ngọc Nguyen (2020), “In vitro Regeneration and Hairy Root Induction of Aconitum carmichaelii ”, SYLWAN, 164(1). ISI Indexed.
2. Nguyen Thi Ngoc Lan, Hoang Thi Thu Hoan, Nguyen Thi Loan, Nguyen Huu Quan, Lo Thi Mai Thu, Sy Danh Thuong, Chu Hoang Mau (2017), “Using ITS and matK barcodes to identify Aconitum samples collected in Quan Ba, Ha Giang, Vietnam ”, Proceedings National Biological Research & Teaching Conference, Quy Nhon 5-2018, pp.1140-1147.
3. Nguyen Thi Ngoc Lan, Tran Thi Hong, Hoang Thi Thu Hoan, Chu Hoang Mau (2018), "In vitro propagation of Aconitum carmichaelii plants", Proceedings National Biotechnology Conference 2018.

Gene sequences on GenBank

1. Hoang,T.T.H., Nguyen,T.N.L., Le,V.S. and Chu,H.M. (2018) Aconitum carmichaelii isolate HSP internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MH410519.1. 19; 06-JUL-2018
2. Hoang,T.T.H., Nguyen,T.N.L., Le,V.S. and Chu,H.M. (2018) Aconitum carmichaelii isolate QB internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MH410520.1. ; 06-JUL-20183. Hoang, T,T.H., Nguyen, T,N.L., Le, V,S and.Chu. and H,M. (2018) Aconitum carmichaelii plastid:chloroplast genomic DNA containing matK gene region, isolate QB, Hagiang. GenBank: LS398143.1. , The Biological master Thesis, Thai Nguyen University of Education, TNU.

5.4. In terms of application

• An in vitro culture procedure has been developed from the stem segments of the Aconitum carmichaelii plants.
• A hairy root culture procedure has been developed from the root segments of the in vitro Aconitum carmichaelii plants.

6. Transfer alternatives, application institutions, impacts and benefits of research results

• The project's products include master theses and articles kept at the Department of Biology, University of Education - Thai Nguyen University in order to be used as materials for learning, teaching and researching in biological field.
• The research results of the project are scientific documents that will be used in teaching thematic subjects for master training and in researching in Biology and Biotechnology at Thai Nguyen University.
• The project contributes to conservation and development of the gene source of aconite plants (Aconitum carmichaeli Debx.) by in vitro culture. This project also supplements scientific information on morphological characteristics and taxonomy of Aconitum carmichaeli in Vietnam.
• The project contributes to in vitro propagation which is foundation for increasing regions of planting aconites in a sustainable manner and conserving of precious medicinal plant gene sources in Vietnam.